Set Windows to
Show Hidden Files and FoldersDo a search for this file
winload16.exe or winload16
It may possibly be in your C:\Windows\System32 folder
Try and delete it if found
If you have trouble deleting it, Try right clicking on the file
Security tab>>advanced>>>Take full control of the file
check the box Allow inheritable permissions
from parent' to propagate... '
If your running XP pro you will have to Disable Simple file sharing
Make sure you also use the search function of XP
When searching click on the Advanced Options
Ensure there is a check in Hidden Files and folders
Stay in safe mode
Open Reg lite
Click Search at the top>>>Search Registry>>Text to Search for--Enter
winload16.exe or just winload16
In the Search in box Enter
RegistryClick the Spyglass at the bottom to begin the search
the entries on the right you can Right click on and jump to the location and Export the key to a folder on your hard drive and then delete the key if it looks malicious
I would also try to download the Stinger from Mcafee and try running it in safe mode
See if it picks up anything
http://vil.nai.com/vil/stinger/